KM+, a mannose-binding lectin from Artocarpus integrifolia: amino acid sequence, predicted tertiary structure, carbohydrate recognition, and analysis of the. The jackfruit, Artocarpus integrifolia (also known as A. heterophyllus), contains a lectin which is specific for D-galactose. The lectin is composed of four α and α’. A simple procedure for extracting a lectin from the seeds of the jackfruit, Artocarpus integrifolia, is described. The extracts had an average protein concentration.
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The jackfruit, Artocarpus integrifolia also known as A.
Its structure and sequences are very similar to those of Maclura pomiferawhich is also a galactose-binding lectin for the family Moraceae. The lectin is also a potent mitogen for T-cells and induces immunoglobulin secretion by B-cells 4. Initially the lectin was used in crude form and was found to bind to human serum proteins, specifically IgA 5.
The specificity of the lectin for IgA is integriffolia to the presence of a terminal galactose residue. Although IgG contains similar sugars, the presence of a terminal sialic acid residue is sufficient to prevent binding of the lectin.
Artocarpus integrifolia – EY Laboratories, Inc.
Jacalin appears to vary in its reactivity with immunoglobulins, depending on the geographic source of the seedspossibly reflecting different isolectin makeup of different genetic pools. Most sources, however, react with IgA and IgD In addition, extracts from artocarlus of Artocarpus altilis breadfruit also exhibit properties similar to jacalin It is therefore important to know the seed source for the lectin before making any conclusions about specificity.
Artocarups, EY Laboratories is supplying a lectin isolated from the Brazilian jackfruit. Immobilized Jacalin is useful in the purification of O-linked glycoproteins While many lectins with a specificity for N-linked glycoproteins have been purified, Jacalin is one of the few lectins to exhibit such a strong preference for Olinked structures.
Jacalin isolated from a Vietnamese str.
Nor does it react with human colon carcinoma also with high metastatic potential However, the interaction appears to be via a protein-protein interaction not involving the carbohydrate binding site of Jacalin nor the glycan moiety of CD4 Tomorrow’s Reagents for Research Today About the Lectin The jackfruit, Artocarpus integrifolia also known as A.
Letter to the Editor — Jacalin: Chaos in its Immunoglobulin- Binding Specificity.
Biology, Biochemistry, Clinical Biochemistry. Product Characteristics Buffer 0.
Artocarpus integrifolia (Jackfruit)
Activity Less than 0. Molecular Weight Normally 65, Da. Caution The purified, lyophilized lectin is not very soluble at neutral pH.
It is recommended that the powder be resuspended in a small quantity of PBS at pH After it has been solubilized, it may artocarpis further diluted with PBS, pH 7. The purified, lyophilized lectin is not very soluble at neutral pH.